Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA–Protein Interactions
نویسندگان
چکیده
منابع مشابه
Identification of surface proteins of Helicobacter pylori by selective biotinylation, affinity purification, and two-dimensional gel electrophoresis.
Helicobacter pylori is a widespread human pathogen that can cause gastric ulcers and cancer. To identify surface proteins that may play a role in pathogen-host interactions and represent potential targets for the control of this infection, we selectively biotinylated intact H. pylori with the hydrophilic reagent sulfosuccinimidyl-6-(biotinamido)-hexanoate and purified the labeled proteins by me...
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ژورنال
عنوان ژورنال: ACS Chemical Biology
سال: 2020
ISSN: 1554-8929,1554-8937
DOI: 10.1021/acschembio.0c00445